RNF8 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77166

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Bovine (92%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all RNF8 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human RNF8. The immunogen is located within amino acids 330 - 380 of RNF8. Amino Acid Squence: MEELNRSKKDFEAI

Specificity

At least three isoforms of RNF8 are known to exist; this antibody will detect all three isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RNF8 Antibody - BSA Free

Western Blot: RNF8 AntibodyBSA Free [NBP1-77166]

Western Blot: RNF8 AntibodyBSA Free [NBP1-77166]

Western Blot: RNF8 Antibody [NBP1-77166] - Human lung tissue lysate with RNF8 antibody at (A) and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: RNF8 Antibody - BSA Free [NBP1-77166]

Immunocytochemistry/ Immunofluorescence: RNF8 Antibody - BSA Free [NBP1-77166]

Immunocytochemistry/Immunofluorescence: RNF8 Antibody [NBP1-77166] - Rat lung tissue with RNF8 antibody at 20 ug/mL.
RNF8 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RNF8 Antibody - BSA Free [NBP1-77166] -

Immunocytochemistry/ Immunofluorescence: RNF8 Antibody - BSA Free [NBP1-77166] - Immunofluorescence of RNF8 in rat lung tissue with RNF8 antibody at 20 u/mL.
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Applications for RNF8 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Western Blot

1-2 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNF8

RNF8 was identified as a ubiquitin ligase (E3) containing a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes including UBE2E1/UBCH6, UBE2E2, and UBE2E3. RNF8 assembles at DNA double-strand breaks (DSBs) via interactions though the FHA domain with the adaptor protein MDC1, resulting in an increase in DSB-associated H2A histone ubiquitinations mediated by the associated ubiquitin ligase RNF168 followed by the accumulation of 53BP1 and BRCA1 repair proteins. Together with RNF168, RNF8 plays an integral part of class switch recombination in B cells, allowing the production of several classes of antibodies, through the recruitment of 53BP1 and BRCA1 to the DSB sites.

Long Name

Ring Finger Protein 8

Alternate Names

ActA Binding Protein 2

Entrez Gene IDs

9025 (Human); 58230 (Mouse); 361815 (Rat)

Gene Symbol

RNF8

UniProt

O76064 (Human)

Additional RNF8 Products

Product Documents for RNF8 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RNF8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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