ROBO2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13364

Novus Biologicals, part of Bio-Techne
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NBP3-13364
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ROBO2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse ROBO2. The exact sequence is proprietary.

Reactivity Notes

Human (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for ROBO2 Antibody

Western Blot: ROBO2 Antibody [NBP3-13364]

Western Blot: ROBO2 Antibody [NBP3-13364]

Western Blot: ROBO2 Antibody [NBP3-13364] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROBO2 antibody (NBP3-13364) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: ROBO2 Antibody [NBP3-13364]

Immunocytochemistry/ Immunofluorescence: ROBO2 Antibody [NBP3-13364]

Immunocytochemistry/Immunofluorescence: ROBO2 Antibody [NBP3-13364] - ROBO2 antibody detects ROBO2 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ROBO2 stained by ROBO2 antibody (NBP3-13364) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: ROBO2 Antibody [NBP3-13364]

Immunohistochemistry-Paraffin: ROBO2 Antibody [NBP3-13364]

Immunohistochemistry-Paraffin: ROBO2 Antibody [NBP3-13364] - ROBO2 antibody detects ROBO2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded neuroepithelium of mouse E10.5 embryo. Green: ROBO2 stained by ROBO2 antibody (NBP3-13364) diluted at 1:500. Red: SOX2, a neural progenitor marker, stained by SOX2 antibody [GT1352] (NBP2-19306) diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for ROBO2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ROBO2

Robo2 belongs to the ROBO family, part of the immunoglobulin superfamily proteins that are highly conserved from fly to human. The encoded protein is a receptor for SLIT2, molecules known to function in axon guidance and cell migration. Defects in this gene are the cause of vesicoureteral reflux type 2. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Long Name

Roundabout Homolog 2

Alternate Names

SAX3

Gene Symbol

ROBO2

Additional ROBO2 Products

Product Documents for ROBO2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ROBO2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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