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ROBO2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25111

Novus Biologicals, part of Bio-Techne
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NBP3-25111

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein ROBO2 using the following amino acid sequence: HLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ROBO2 Antibody

ROBO2 Antibody Immunocytochemistry/Immunofluorescence: ROBO2 Antibody [NBP3-25111]

Immunocytochemistry/Immunofluorescence: ROBO2 Antibody [NBP3-25111]

Staining of human cell line HEL shows localization to nucleoplasm, plasma membrane & centriolar satellites.

Applications for ROBO2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 µg/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ROBO2

Robo2 belongs to the ROBO family, part of the immunoglobulin superfamily proteins that are highly conserved from fly to human. The encoded protein is a receptor for SLIT2, molecules known to function in axon guidance and cell migration. Defects in this gene are the cause of vesicoureteral reflux type 2. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Long Name

Roundabout Homolog 2

Alternate Names

SAX3

Gene Symbol

ROBO2

Additional ROBO2 Products

Product Documents for ROBO2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ROBO2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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