ROCK1 Antibody (2E2)

Novus Biologicals, part of Bio-Techne | Catalog # H00006093-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ROCK1 Antibodies »

Product Specifications

Immunogen

ROCK1 (NP_005397, 401 a.a. ~ 510 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRR

Localization

Cytoplasmic, Golgi Apparatus and Plasma membrane

Specificity

ROCK1 - Rho-associated, coiled-coil containing protein kinase 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ROCK1 Antibody (2E2)

Western Blot: ROCK1 Antibody (2E2) [H00006093-M01]

Western Blot: ROCK1 Antibody (2E2) [H00006093-M01]

Western Blot: ROCK1 Antibody (2E2) [H00006093-M01] - ROCK1 monoclonal antibody (M01), clone 2E2 Analysis of ROCK1 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: ROCK1 Antibody (2E2) [H00006093-M01]

Immunocytochemistry/ Immunofluorescence: ROCK1 Antibody (2E2) [H00006093-M01]

Immunocytochemistry/Immunofluorescence: ROCK1 Antibody (2E2) [H00006093-M01] - Analysis of monoclonal antibody to ROCK1 on HeLa cell. Antibody concentration 10 ug/ml.
ELISA: ROCK1 Antibody (2E2) [H00006093-M01]

ELISA: ROCK1 Antibody (2E2) [H00006093-M01]

ELISA: ROCK1 Antibody (2E2) [H00006093-M01] - ROCK1 Antibody (2E2) [H00006093-M01] - Detection limit for recombinant GST tagged ROCK1 is approximately 0.03ng/ml as a capture antibody.

Applications for ROCK1 Antibody (2E2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ROCK1

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity.

Long Name

Rho-associated, Coiled-Coil Containing Protein Kinase 1

Alternate Names

p160ROCK, ROK beta

Entrez Gene IDs

6093 (Human)

Gene Symbol

ROCK1

OMIM

601702 (Human)

UniProt

NP_005397 (Human)

Additional ROCK1 Products

Product Documents for ROCK1 Antibody (2E2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ROCK1 Antibody (2E2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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