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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Rabbit (93%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ROCK1. The immunogen is located within the first 50 amino acids of ROCK1. Amino Acid Squence: DSFETRFEKMDNLLR

Specificity

ROCK1 antibody is predicted to not cross-react with other ROCK protein family members.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ROCK1 Antibody - BSA Free

Western Blot: ROCK1 AntibodyBSA Free [NBP1-76569]

Western Blot: ROCK1 AntibodyBSA Free [NBP1-76569]

Western Blot: ROCK1 Antibody [NBP1-76569] - 293 cell lysate with ROCK1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: ROCK1 Antibody - BSA Free [NBP1-76569]

Immunocytochemistry/ Immunofluorescence: ROCK1 Antibody - BSA Free [NBP1-76569]

Immunocytochemistry/Immunofluorescence: ROCK1 Antibody [NBP1-76569] - Immunocytochemistry of ROCK1 in 293 cells with ROCK1 antibody at 10 ug/mL.
Immunocytochemistry/ Immunofluorescence: ROCK1 Antibody - BSA Free [NBP1-76569]

Immunocytochemistry/ Immunofluorescence: ROCK1 Antibody - BSA Free [NBP1-76569]

Immunocytochemistry/Immunofluorescence: ROCK1 Antibody [NBP1-76569] - Immunofluorescence of ROCK1 in 293 cells with ROCK1 antibody at 20 ug/mL.

Applications for ROCK1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ROCK1

ROCK1 is a member of the AGC serine/threonine protein kinase family that regulates the assembly of the actin cytoskeleton. ROCK1 contains a protein kinase domain, a coiled-coil region and a zinc finger region and appears to be present as a dimer. ROCK1, like its isozyme ROCK2, is a downstream target of the small G-protein Rho and plays a role in smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, and mediates the cellular morphological changes during apoptosis.

Long Name

Rho-associated, Coiled-Coil Containing Protein Kinase 1

Alternate Names

p160ROCK, ROK beta

Gene Symbol

ROCK1

Additional ROCK1 Products

Product Documents for ROCK1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ROCK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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