Roquin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-656
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1075 and the C-terminus (residue 1133) of human Ring Finger and CCCH-type finder domains 1 using the numbering given in entry NP_742068.1 (GeneID 149041).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Roquin Antibody
![Western Blot: Roquin Antibody [NB100-656]](https://resources.bio-techne.com/images/products/Roquin-Antibody-Western-Blot-NB100-656-img0001.jpg "Western Blot: Roquin Antibody [NB100-656]")
Western Blot: Roquin Antibody [NB100-656]
Western Blot: Roquin Antibody [NB100-656] - Samples: A) Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T, 50 ug) cells. B) Whole cell lysate (1 mg/IP; 1/4 of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Roquin antibody NB100-656 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP (B). Roquin was also immunoprecipitated using rabbit anti-Roquin antibody NB100-655 at 3 ug/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for Roquin Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:1000-1:10000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Roquin
Alternate Names
KIAA2025RP5-1198E17.5, RING finger and C3H zinc finger protein 1, ring finger and CCCH-type domains 1, RING finger protein 198, RNF198ring finger and CCCH-type zinc finger domains 1, ROQUIN
Entrez Gene IDs
149041 (Human)
Gene Symbol
RC3H1
UniProt
Additional Roquin Products
Product Documents for Roquin Antibody
Product Specific Notices for Roquin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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