ROR alpha/NR1F1 Antibody

Western Blot: ROR alpha/NR1F1 Antibody [NBP1-52813] -

Western Blot: ROR alpha/NR1F1 Antibody [NBP1-52813] - BMAL1 & ROR alpha do not bind to HIF-1 alpha in NP cellsA. Immunoprecipitation (IP) of BMAL1 & CLOCK from NP cells cultured under normoxia or hypoxia for 24 h followed by Western blot analysis using anti-HIF-1 alpha, anti-BMAL1 & anti-CLOCK antibodies. BMAL1 bound to CLOCK, but neither BMAL1 nor CLOCK bound to HIF-1 alpha irrespective of oxygen tension. Preimmune rabbit IgG was used as a negative control for IP assays. B. Pulldown of HIF-1 alpha did not show co-precipitation of BMAL1, CLOCK, or ROR alpha. HC: heavy chain of IgG, nsp: non-specific (C) Pulldown of HIF-1 alpha showed co- precipitation of HIF-1 beta/ARNT, association was higher under hypoxia. D., E. Treatment of NP cells with ROR alpha inhibitor, ML-176 (10 μM), showed no effect on nuclear levels of HIF-1 alpha. Densitometric analysis shown in (E) was performed on blots from 3 independent experiments, Data is represented as mean ± SE, * p < 0.05. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27049729), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ROR alpha/NR1F1 Antibody [NBP1-52813] -

Western Blot: ROR alpha/NR1F1 Antibody [NBP1-52813] - Expression analysis of BMAL1 & other related factors in NP cellsA. Immunohistochemical localization of BMAL1 in rat intervertebral disc. Sagittal sections of the mature rat intervertebral disc, immunostained with BMAL1 antibody, showed prominent nuclear expression in NP tissue. B. Western blot analysis of BMAL1 & ROR alpha expression in NP tissues isolated from three rats showed positive expression for both the proteins. C. qRT-PCR analysis of BMAL-1 & ROR alpha mRNA expression from NP & AF tissues from rat discs (n=3 animals/group) D. qRT-PCR analysis of BMAL1, ROR alpha, ARNT, ARNT2, ARNTL2 & CLOCK expression in rat NP cells cultured under hypoxia (1% O2). None of the genes showed significant increase in hypoxia. E. Western blot analysis of BMAL1 & ROR alpha in NP cells cultured under hypoxia. F., G. Densitometric analysis of multiple blots shown in (E) above. No significant differences were seen between normoxic & hypoxic levels of BMAL1 & ROR alpha. Data is represented as mean ± SE, n=3, p<0.05. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27049729), licensed under a CC-BY license. Not internally tested by Novus Biologicals.