ROR gamma/RORC/NR1F3 Antibody (4G419) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80930

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4G419

Format

Azide and BSA Free

Concentration

1 mg/ml

View all ROR gamma/RORC/NR1F3 Antibodies »

Product Summary for ROR gamma/RORC/NR1F3 Antibody (4G419) - Azide and BSA Free

Immunogen

A portion of amino acids 1-50 of human ROR gamma was used as the immunogen.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 22110656).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for ROR gamma/RORC/NR1F3 Antibody (4G419) - Azide and BSA Free

Western Blot: ROR gamma/RORC/NR1F3 Antibody (4G419)Azide and BSA Free [NBP2-80930]

Western Blot: ROR gamma/RORC/NR1F3 Antibody (4G419)Azide and BSA Free [NBP2-80930]

Western Blot: ROR gamma/RORC/NR1F3 Antibody (4G419) - Azide and BSA Free [NBP2-80930] - Analysis in (A) human thymus lysate, (B) transfected 293 cell lysate, and (C) untransfected 293 cell lysate. Dilution: 5 ug/ml. Image from the standard format of this antibody.
Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) - Azide and BSA Free [NBP2-80930]

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) - Azide and BSA Free [NBP2-80930]

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) - Azide and BSA Free [NBP2-80930] - Intracellular analysis of ROR gamma in human lymphocytes using 0.25 ug of this antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody ; red represents ROR gamma antibody. Image from the standard format of this antibody.
Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) - Azide and BSA Free [NBP2-80930]

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) - Azide and BSA Free [NBP2-80930]

Flow Cytometry: ROR gamma/RORC/NR1F3 Antibody (4G419) - Azide and BSA Free [NBP2-80930] - Analysis using the Allophycocyanin conjugate of NBP2-24449. Staining of ROR gamma in human lymphocytes using 10 ul (0.25 ug) of ROR gamma antibody. Shaded histogram represents cells without antibody; green represents isotype control antibodywas used for this test.
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Applications for ROR gamma/RORC/NR1F3 Antibody (4G419) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.25-0.5 ug/10^6 cells

Immunohistochemistry

reported in scientific literature (PMID 30671063)

Western Blot

5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ROR gamma/RORC/NR1F3

ROR gamma/RORC/NR1F3, aka RAR Orphan Receptor gamma, is a Nuclear Receptor 1 Thyroid Hormone-Like receptor containing a short A-T rich sequence follow by a single core motif half-site 5'-AGGTCA-3'. RORC is a key regulator of thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. NR1F3 regulates intrinsic transcriptional functions. Unlike other major nuclear receptors, RORC binds as a monomer to hormone response elements and is documented in mouse thymus, adipose, bone, skeletal muscle, liver, kidney, and human skeletal muscle. An N-terminal isoform of ROR gamma, ROR gamma T, inhibits Fas ligand expression and cytokine secretion in immature thymocytes.

Long Name

Retinoic Acid-Related Orphan Receptor gamma

Alternate Names

NR1F3, RORC, RZRG

Gene Symbol

RORC

Additional ROR gamma/RORC/NR1F3 Products

Product Documents for ROR gamma/RORC/NR1F3 Antibody (4G419) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ROR gamma/RORC/NR1F3 Antibody (4G419) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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