ROR gamma/RORC/NR1F3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24503

Novus Biologicals, part of Bio-Techne
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NBP2-24503SS
NBP2-24503
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Orangutan (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all ROR gamma/RORC/NR1F3 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 1-50 of human ROR gamma/RORC/NR1F3 was used as the immunogen.

Reactivity Notes

The amino acid sequence used as immunogen Porcine, Rat (81%).

Specificity

100% homologous in human isoforms CRA_a).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ROR gamma/RORC/NR1F3 Antibody - BSA Free

Western Blot: ROR gamma/RORC/NR1F3 AntibodyBSA Free [NBP2-24503]

Western Blot: ROR gamma/RORC/NR1F3 AntibodyBSA Free [NBP2-24503]

Western Blot: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Total protein from Human and mouse Thymus, Jurkat and U2OS cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ROR Gamma in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: ROR gamma/RORC/NR1F3 Antibody - BSA Free [NBP2-24503]

Immunocytochemistry/ Immunofluorescence: ROR gamma/RORC/NR1F3 Antibody - BSA Free [NBP2-24503]

Immunocytochemistry/Immunofluorescence: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - U-2 OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ROR gamma overnight at 4C and detected with an anti-rabbit Dylight 488 (Green). Alpha tubulin (DM1A) NB100-690 was used as a co-stain and detected with an anti-mouse Dylight 550 (Red). Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody - BSA Free [NBP2-24503]

Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody - BSA Free [NBP2-24503]

Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Tissue section of human lymph node using anti- ROR gamma/RORC/NR1F3 antibody. The staining was developed with HRP labeled secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This antibody generated mainly a nuclear staining in a subset of cells and a weak to cytoplasmic staining was also observed in some cells.
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Applications for ROR gamma/RORC/NR1F3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

2.5-10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

reported in scientific literature (PMID 31451788)

Western Blot

0.5-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-24503 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ROR gamma/RORC/NR1F3

ROR gamma/RORC/NR1F3, aka RAR Orphan Receptor gamma, is a Nuclear Receptor 1 Thyroid Hormone-Like receptor containing a short A-T rich sequence follow by a single core motif half-site 5'-AGGTCA-3'. RORC is a key regulator of thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. NR1F3 regulates intrinsic transcriptional functions. Unlike other major nuclear receptors, RORC binds as a monomer to hormone response elements and is documented in mouse thymus, adipose, bone, skeletal muscle, liver, kidney, and human skeletal muscle. An N-terminal isoform of ROR gamma, ROR gamma T, inhibits Fas ligand expression and cytokine secretion in immature thymocytes.

Long Name

Retinoic Acid-Related Orphan Receptor gamma

Alternate Names

NR1F3, RORC, RZRG, anti-ROR gamma

Gene Symbol

RORC

UniProt

NP_001009598 (Human)

Additional ROR gamma/RORC/NR1F3 Products

Product Documents for ROR gamma/RORC/NR1F3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ROR gamma/RORC/NR1F3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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