ROR gamma t/RORC2/NR1F3 Antibody (PSH04-38)

Western Blot: ROR gamma t/RORC2/NR1F3 Antibody (PSH04-38) [NBP3-32915]

Western Blot: ROR gamma t/RORC2/NR1F3 Antibody (PSH04-38) [NBP3-32915] - Western blot analysis of ROR gamma t/RORC2/NR1F3 on different lysates with Rabbit anti-ROR gamma t/RORC2/NR1F3 antibody (NBP3-32915) at 1/2,000 dilution.

Lane 1: HCT 116 cell lysate
Lane 2: Rat thymus tissue lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 58 kDa
Observed band size: 55 kDa

Exposure time: 3 minutes; ECL;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32915) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: ROR gamma t/RORC2/NR1F3 Antibody (PSH04-38) [NBP3-32915]

Immunohistochemistry: ROR gamma t/RORC2/NR1F3 Antibody (PSH04-38) [NBP3-32915] - Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-ROR gamma t/RORC2/NR1F3 antibody (NBP3-32915) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32915) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.