Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1A1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ROS1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1882-2125 of human ROS1(NP_002935) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for ROS Antibody (OTI1A1) - Azide and BSA Free

Western Blot: ROS Antibody (OTI1A1)Azide and BSA Free [NBP2-73933]

Western Blot: ROS Antibody (OTI1A1)Azide and BSA Free [NBP2-73933]

Western Blot: ROS Antibody (OTI1A1) - Azide and BSA Free [NBP2-73933] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROS.
Immunohistochemistry: ROS Antibody (OTI1A1) - Azide and BSA Free [NBP2-73933]

Immunohistochemistry: ROS Antibody (OTI1A1) - Azide and BSA Free [NBP2-73933]

Immunohistochemistry: ROS Antibody (OTI1A1) - Azide and BSA Free [NBP2-73933] - Staining of HCC78 cells using anti-ROS1 mouse monoclonal antibody (Left). The right is HELA cells as negative control.
Immunohistochemistry: ROS Antibody (OTI1A1) - Azide and BSA Free [NBP2-73933]

Immunohistochemistry: ROS Antibody (OTI1A1) - Azide and BSA Free [NBP2-73933]

Immunohistochemistry: ROS Antibody (OTI1A1) - Azide and BSA Free [NBP2-73933] - Staining of paraffin-embedded HCC78 (left) and HeLa (right) xenograft using TA805804 (0.2ug/ml). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3min).

Applications for ROS Antibody (OTI1A1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:5000-10000

Immunohistochemistry

1:500

Western Blot

1:200 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ROS1

This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. (provided by RefSeq)

Long Name

ROS Proto-Oncogene 1, Receptor Tyrosine Kinase

Alternate Names

c-Ros-1, MCF3

Gene Symbol

ROS1

Additional ROS1 Products

Product Documents for ROS Antibody (OTI1A1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ROS Antibody (OTI1A1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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