RPA14 Antibody (1F4)

Novus Biologicals, part of Bio-Techne | Catalog # H00006119-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Lambda Clone # 1F4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RPA14 Antibodies »

Product Specifications

Immunogen

RPA3 (AAH05264, 12 a.a. ~ 121 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. INAGMLAQFIDKPVCFVGRLEKIHPTGKMFILSDGEGKNGTIELMEPLDEEISGIVEVVGRVTAKATILCTSYVQFKEDSHPFDLGLYNEAVKIIHDFPQFYPLGIVQHD

Localization

Nuclear

Specificity

RPA3 - replication protein A3, 14kDa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 Lambda

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RPA14 Antibody (1F4)

Western Blot: RPA14 Antibody (1F4) [H00006119-M01]

Western Blot: RPA14 Antibody (1F4) [H00006119-M01]

Western Blot: RPA14 Antibody (1F4) [H00006119-M01] - Analysis of RPA3 expression in transfected 293T cell line by RPA3 monoclonal antibody (M01), clone 1F4.Lane 1: RPA3 transfected lysate(13.6 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: RPA14 Antibody (1F4) [H00006119-M01]

Immunohistochemistry-Paraffin: RPA14 Antibody (1F4) [H00006119-M01]

Immunohistochemistry-Paraffin: RPA14 Antibody (1F4) [H00006119-M01] - Analysis of monoclonal antibody to RPA3 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.
Western Blot: RPA14 Antibody (1F4) [H00006119-M01]

Western Blot: RPA14 Antibody (1F4) [H00006119-M01]

Western Blot: RPA14 Antibody (1F4) [H00006119-M01] - Analysis of RPA3 over-expressed 293 cell line, cotransfected with RPA3 Validated Chimera RNAi ( Cat # H00006119-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RPA3 monoclonal antibody (M01), clone 1F4 (Cat # H00006119-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Applications for RPA14 Antibody (1F4)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RPA14

Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase.

Alternate Names

REPA3Replication factor A protein 3, replication protein A 14 kDa subunit, replication protein A3 (14kD), replication protein A3, 14kDa, RF-A protein 3, RP-A p14, RPA14

Entrez Gene IDs

6119 (Human)

Gene Symbol

RPA3

OMIM

179837 (Human)

UniProt

AAH05264 (Human)

Additional RPA14 Products

Product Documents for RPA14 Antibody (1F4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RPA14 Antibody (1F4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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