RPA2 [p Ser33] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-544

Novus Biologicals, part of Bio-Techne
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NB100-544
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Cited:

Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RPA2 Antibodies »

Product Specifications

Immunogen

Immunogen was a phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serine that corresponded to position 33 using the numbering given in entry NP_002937.1 (GeneID 6118).

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33087072).

Modification

p Ser33

Specificity

NB100-544 is specific for human RPA-32 pSer33 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPA2 [p Ser33] Antibody

Western Blot: RPA2 [p Ser33] Antibody [NB100-544]

Western Blot: RPA2 [p Ser33] Antibody [NB100-544]

Western Blot: RPA2 [p Ser33] Antibody [NB100-544] - Whole cell lysate (50 ug) from HeLa cells treated with 100 uM etoposide for 16 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho RPA32 (S33) antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower panel: Rabbit anti-RPS3 antibody.
Immunocytochemistry/ Immunofluorescence: RPA2 [p Ser33] Antibody [NB100-544]

Immunocytochemistry/ Immunofluorescence: RPA2 [p Ser33] Antibody [NB100-544]

Immunocytochemistry/Immunofluorescence: RPA2 [p Ser33] Antibody [NB100-544] - Active DNA end processing in late micronuclei compartments. Representative images (Upper panel) and quantification of RPA signal in MN compartments (Lower left) or RPA foci in nuclei (lower right) of post-IR OVCAR-8 cells after indicated treatments. Pro-resection genes (MRE11, BRCA1 and WDR70) were knockdown by specific siRNA. Percentage of positive RPA2-pS33 MN population was calculated after 36 h of IR treatment Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/s12885-018-4347-0), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: RPA2 [p Ser33] Antibody [NB100-544]

Immunocytochemistry/ Immunofluorescence: RPA2 [p Ser33] Antibody [NB100-544]

Immunocytochemistry/Immunofluorescence: RPA2 [p Ser33] Antibody [NB100-544] - L.36 PDAC cells were treated two hours using 5 mM hydroxyurea to induce RPA32 Ser33 phosphorylation. The antibody produces clear nuclear staining of treated cells. ICC/IF image submitted by a verified customer review.
View 1 more images

Applications for RPA2 [p Ser33] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500 - 1:2500

Western Blot

1:1000 - 1:5000
Application Notes
Numerous bands representing proteins that migrate more slowly than 60 to 70 kDA have been observed during WB. Under some conditions, at least two of these may be more intense than the band that represents phospho RPA32-S33. ICC/IF reactivity reported in (PMID: 27723720).
See Simple Western Antibody Database for Simple Western validation: tested in Human squamous carcinoma cells; antibody dilution of 1:25; separated by charge
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB100-544 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RPA2

Replication Protein A (RPA) is a single stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 14, 32 and 70kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA is a target for DNA dependent protein kinase (DNA-PK), Ataxia Telangiectasia Mutated gene (ATM) and a cyclin dependent protein kinase. The middle subunit, RPA 32, is phosphorylated during the S-phase of the cell cycle in response to DNA damage, and during apoptosis of Jurkat T-lymphocytes.

Long Name

Replication Protein A2, 32kDa

Alternate Names

REPA2, RPA32

Entrez Gene IDs

6118 (Human)

Gene Symbol

RPA2

UniProt

P15927 (Human)

Additional RPA2 Products

Product Documents for RPA2 [p Ser33] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RPA2 [p Ser33] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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