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RPA2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89524

Novus Biologicals, part of Bio-Techne
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NBP1-89524
NBP1-89524-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for RPA2 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HMVLSKANSQPSAGRAPISNPGMSEAGNFGGNSFMPANGLTVAQNQVLNLIKACPRPEGLNFQDLKNQLKHMSVSSIKQAVDFLSNEGHIYSTVDDDH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPA2 Antibody

Western Blot: RPA2 Antibody [NBP1-89524]

Western Blot: RPA2 Antibody [NBP1-89524]

Western Blot: RPA2 Antibody [NBP1-89524] - Analysis using Anti-RPA2 antibody NBP1-89524 (A) shows similar pattern to independent antibody NBP1-89523 (B).
Immunocytochemistry/ Immunofluorescence: RPA2 Antibody [NBP1-89524]

Immunocytochemistry/ Immunofluorescence: RPA2 Antibody [NBP1-89524]

Immunocytochemistry/Immunofluorescence: RPA2 Antibody [NBP1-89524] - Staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524]

Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524]

Immunohistochemistry-Paraffin: RPA2 Antibody [NBP1-89524] - Staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts and Leydig cells.

Applications for RPA2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000-1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPA2

RPA2 is required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions

Long Name

Replication Protein A2, 32kDa

Alternate Names

REPA2, RPA32

Gene Symbol

RPA2

Additional RPA2 Products

Product Documents for RPA2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RPA2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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