RPA70 Antibody (5R7S3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16174

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16174-100ul
NBP3-16174-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5R7S3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RPA70 Antibodies »

Product Summary for RPA70 Antibody (5R7S3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RPA70 (P27694). MVGQLSEGAIAAIMQKGDTNIKPILQVINIRPITTGNSPPRYRLLMSDGLNTLSSFMLATQLNPLVEEEQLSSNCVCQIHRFIVNTLKDGRRVVILMELE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPA70 Antibody (5R7S3)

Western Blot: RPA70 Antibody (5R7S3) [NBP3-16174]

Western Blot: RPA70 Antibody (5R7S3) [NBP3-16174]

Western Blot: RPA70 Antibody (5R7S3) [NBP3-16174] - Western blot analysis of extracts of various cell lines, using RPA70 Rabbit mAb (NBP3-16174) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunoprecipitation: RPA70 Antibody (5R7S3) [NBP3-16174]

Immunoprecipitation: RPA70 Antibody (5R7S3) [NBP3-16174]

Immunoprecipitation: RPA70 Antibody (5R7S3) [NBP3-16174] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug RPA70 antibody (NBP3-16174). Western blot was performed from the immunoprecipitate using RPA70 antibody (NBP3-16174) at a dilition of 1:1000.

Applications for RPA70 Antibody (5R7S3)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:500-1:1000

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RPA70

RPA (Replication protein A) is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). RPA is a single strand binding protein that is involved in replication, homologous recombination and nucleotide excision and repair. It colocalises with RAD51 on synapsed axes in meiosis, facilitating RAD51 function. Replication protein A acts as an auxiliary protein for DNA polymerases alpha and delta.

Alternate Names

HSSB, MST075, MSTP075, REPA1RF-A protein 1, Replication factor A protein 1, replication protein A 70 kDa DNA-binding subunit, replication protein A1 (70kD), replication protein A1, 70kDa, RF-A, RP-A, RPA70RP-A p70, Single-stranded DNA-binding protein

Gene Symbol

RPA1

Additional RPA70 Products

Product Documents for RPA70 Antibody (5R7S3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RPA70 Antibody (5R7S3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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