RPB2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93593

Novus Biologicals, part of Bio-Techne
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NBP2-93593-0.02ml
NBP2-93593-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RPB2 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2B (NP_000929.1). MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPB2 Antibody - BSA Free

Western Blot: RPB2 AntibodyBSA Free [NBP2-93593]

Western Blot: RPB2 AntibodyBSA Free [NBP2-93593]

Western Blot: RPB2 Antibody [NBP2-93593] - Analysis of extracts of various cell lines, using RPB2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.
Immunohistochemistry-Paraffin: RPB2 Antibody - BSA Free [NBP2-93593]

Immunohistochemistry-Paraffin: RPB2 Antibody - BSA Free [NBP2-93593]

Immunohistochemistry-Paraffin: RPB2 Antibody [NBP2-93593] - Paraffin-embedded rat liver using RPB2 .
Immunohistochemistry-Paraffin: RPB2 Antibody - BSA Free [NBP2-93593]

Immunohistochemistry-Paraffin: RPB2 Antibody - BSA Free [NBP2-93593]

Immunohistochemistry-Paraffin: RPB2 Antibody [NBP2-93593] - Paraffin-embedded mouse liver using RPB2 .
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Applications for RPB2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RPB2

It is suggested that Rpb2, a 140 kDa protein, plays a role is enhancing transcription from prostate specific antigen genes. These genes may be linked to prostate cancer progression.

Alternate Names

DNA-directed RNA polymerase II subunit RPB2, EC 2.7.7, EC 2.7.7.6, hRPB140, hsRPB2, POL2RB, polymerase (RNA) II (DNA directed) polypeptide B (140kD), polymerase (RNA) II (DNA directed) polypeptide B, 140kDa, RNA polymerase II subunit 2, RNA polymerase II subunit B2, RNA polymerase II subunit2, RNA polymerase II second largest subunit

Gene Symbol

POLR2B

Additional RPB2 Products

Product Documents for RPB2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RPB2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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