RPB9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92344

Novus Biologicals, part of Bio-Techne
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NBP1-92344
NBP1-92344-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RPB9 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PDGTYEPGFVGIRFCQECNNMLYPKEDKENRILLYACRNCDYQQEADNSCIYVNKITHEVDELTQIIADVSQDPTLPRTEDHPCQKCGHKEAVFFQSHSA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPB9 Antibody

Western Blot: RPB9 Antibody [NBP1-92344]

Western Blot: RPB9 Antibody [NBP1-92344]

Western Blot: RPB9 Antibody [NBP1-92344] - Analysis in human cell line HEK 293.
Immunocytochemistry/ Immunofluorescence: RPB9 Antibody [NBP1-92344]

Immunocytochemistry/ Immunofluorescence: RPB9 Antibody [NBP1-92344]

Immunocytochemistry/Immunofluorescence: RPB9 Antibody [NBP1-92344] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: RPB9 Antibody [NBP1-92344]

Immunohistochemistry-Paraffin: RPB9 Antibody [NBP1-92344]

Immunohistochemistry-Paraffin: RPB9 Antibody [NBP1-92344] - Staining of human cervix, uterine shows moderate nucleolus positivity in squamous epithelial cells.
View 1 more images

Applications for RPB9 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPB9

RPB9 encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [provided by RefSeq]

Alternate Names

DNA-directed RNA polymerase II 14.5 kDa polypeptide, DNA-directed RNA polymerase II subunit I, DNA-directed RNA polymerase II subunit RPB9, hRPB14.5, polymerase (RNA) II (DNA directed) polypeptide I (14.5kD), polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa, RNA polymerase II 14.5 kDa subunit, RNA polymerase II subunit B9, RPB14.5, RPB9

Gene Symbol

POLR2I

Additional RPB9 Products

Product Documents for RPB9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RPB9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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