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RPL29 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49581

Novus Biologicals, part of Bio-Techne
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NBP2-49581
NBP2-49581-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for RPL29 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PASVPAQAPKRTQAPTKASE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPL29 Antibody

Western Blot: RPL29 Antibody [NBP2-49581]

Western Blot: RPL29 Antibody [NBP2-49581]

Western Blot: RPL29 Antibody [NBP2-49581] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: RPL29 Antibody [NBP2-49581]

Immunocytochemistry/ Immunofluorescence: RPL29 Antibody [NBP2-49581]

Immunocytochemistry/Immunofluorescence: RPL29 Antibody [NBP2-49581] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli, cytosol & endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RPL29 Antibody [NBP2-49581]

Immunohistochemistry-Paraffin: RPL29 Antibody [NBP2-49581]

Immunohistochemistry-Paraffin: RPL29 Antibody [NBP2-49581] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells and glial cells.

Applications for RPL29 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPL29

RPL29, also known as 60S ribosomal protein L29, is a 18kDa 159 amino acid protein and belongs to the L29E family of ribosomal proteins. The primary function of RPL29 is to encode a cytoplasmic ribosomal protein. Current research is being conducted on RPL29 and its relationship with the diseases colon cancer, thyroid carcinoma, lung carcinoma, colorectal cancer, thyroiditis, carcinoma, breast cancer and malaria. RPL29 has been linked to the ossification, localization, pathogenesis, cell adhesion and cell proliferation pathways where it interacts with UBC, SYBU, CHD3, RPL24 and RPL6.

Alternate Names

60S ribosomal protein L29, Cell surface heparin-binding protein HIP, heparin/heparan sulfate-binding protein, heparin/heparan sulfate-interacting protein, HIPHUMRPL29, HP/HS-interacting protein, MGC88589, ribosomal protein L29

Gene Symbol

RPL29

Additional RPL29 Products

Product Documents for RPL29 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RPL29 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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