RPL3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81332

Novus Biologicals, part of Bio-Techne
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NBP1-81332
NBP1-81332-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RPL3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EDGKKQLEKDFSSMKKYCQVIRVIAHTQMRLLPLRQKKAHLMEIQVNGGTVAEKLDWARERLEQQVPVNQVFGQDEMIDVIGVTKGKGYKGVTSRWHTKKLPRKTHR

Reactivity Notes

Reactivity reported in scientific literature (PMID: 19454283)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPL3 Antibody

Western Blot: RPL3 Antibody [NBP1-81332]

Western Blot: RPL3 Antibody [NBP1-81332]

Western Blot: RPL3 Antibody [NBP1-81332] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: RPL3 Antibody [NBP1-81332]

Immunocytochemistry/ Immunofluorescence: RPL3 Antibody [NBP1-81332]

Immunocytochemistry/Immunofluorescence: RPL3 Antibody [NBP1-81332] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RPL3 Antibody [NBP1-81332]

Immunohistochemistry-Paraffin: RPL3 Antibody [NBP1-81332]

Immunohistochemistry-Paraffin: RPL3 Antibody [NBP1-81332] - Staining of human cerebral cortex shows strong cytoplasmic and nuclear(nucleolar) positivity in neuronal cells.

Applications for RPL3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPL3

RPL3, also known as 60S ribosomal protein L3, is a 46 kDa 403 amino acid protein found in the cytoplasm. The primary function of RPL3 is believed to contribute to tat-mediated transactivation. Research is currently being conducted on RPL3 in relation to Treacher Collins syndrome, kidney disease, esophageal carcinoma, ovarian carcinoma, esophagitis, colon cancer, prostate cancer, carcinoma, prostatitis and malaria. The gene has been linked to the pathogenesis, innervation, translation, transport and metabolism of mRNA pathways where it interacts with RPS3, RPL4, RPL8, RPL11, and RPL12.

Long Name

60S ribosomal protein L3

Alternate Names

OK/SW-cl.32, TARBP-B

Gene Symbol

RPL3

Additional RPL3 Products

Product Documents for RPL3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RPL3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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