RPL35 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15546
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 50-120 of human RPL35 (NP_009140.1). VRKSIARVLTVINQTQKENLRKFYKGKKYKPLDLRPKKTRAMRRRLNKHEENLKTKKQQRKERLYPLRKYA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RPL35 Antibody - Azide and BSA Free
Western Blot: RPL35 AntibodyAzide and BSA Free [NBP3-15546]
Western Blot: RPL35 Antibody [NBP3-15546] - Western blot analysis of extracts of various cell lines, using RPL35 Rabbit pAb (NBP3-15546) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Immunocytochemistry/ Immunofluorescence: RPL35 Antibody - Azide and BSA Free [NBP3-15546]
Immunocytochemistry/Immunofluorescence: RPL35 Antibody [NBP3-15546] - Immunofluorescence analysis of L929 cells using RPL35 antibody (NBP3-15546) at dilution of 1:100. Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: RPL35 Antibody - Azide and BSA Free [NBP3-15546]
Immunohistochemistry-Paraffin: RPL35 Antibody [NBP3-15546] - Immunohistochemistry of paraffin-embedded Human colon carcinoma using RPL35 Rabbit pAb (NBP3-15546) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for RPL35 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 50% glycerol, pH7.3
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RPL35
Alternate Names
M, ribosomal protein L35,60S ribosomal protein L35
Gene Symbol
RPL35
Additional RPL35 Products
Product Documents for RPL35 Antibody - Azide and BSA Free
Product Specific Notices for RPL35 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov