RPL5 Antibody

Western Blot: RPL5 Antibody [NBP1-31413]

Western Blot: RPL5 Antibody [NBP1-31413] - A. 30 ug Rat2 whole cell lysate/extract 10 % SDS-PAGE RPL5 antibody dilution: 1:1000

Western Blot: RPL5 Antibody [NBP1-31413]

Western Blot: RPL5 Antibody [NBP1-31413] - A: Mouse brain 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: RPL5 Antibody [NBP1-31413]

Immunohistochemistry-Paraffin: RPL5 Antibody [NBP1-31413] - Gastric CA N87 xenograft, using RPL5 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Knockdown Validated: RPL5 Antibody [NBP1-31413]

Knockdown Validated: RPL5 Antibody [NBP1-31413] - Western Blot analysis of RPL5 protein expressions in CD71highTer119high cells. There was a significant decrease in the expression level of RPL5 in the treated shRNA Rpl5 compared to the control mice. Image collected and cropped by CiteAb from the following publication (www.discoveriesjournals.org/discoveries/D.2019.03.DI-Kazerounian-Beggs.DOI) licensed under a CC-BY license.

Western Blot: RPL5 Antibody [NBP1-31413] -

Western Blot: RPL5 Antibody [NBP1-31413] - A. Methylcellulose colony assay on bone marrow cells from doxycycline treated shRNA Rpl5 & control mice (n=3), (CFU-E, P=0.0003; BFU-E, P=1; GFU-GM, P=0.3); B. FACS analysis of bone marrow cells from shRNA Rpl5 & control mice. Total bone marrow cells from shRNA Rpl5 (B.1) & control (B.2) mice; viable cells were gated for analysis & sorting. We used the cell-surface markers CD71 (Y-axis) & Ter119 (X-axis) to separate mature erythroids & erythroid precursors. The most mature erythroblasts are CD71lowTer119high, while the erythroid precursors are CD71highTer119intermediate cells. In our FACS analysis the gated population comprises CD71highTer119med cells (P4) & CD71highTer119high cells (P5) from shRNA Rpl5 (B.3 & B.5) & control (B4 & B6) mice; the results here are collected from one mouse per group & are representative of 6 independent experiments; in each experiment, cells were collected from 1-3 mice for each group & sorted independently; C. Western Blot analysis of RPL5 protein expressions in CD71highTer119high cells. There was a significant decrease in the expression level of RPL5 in the treated shRNA Rpl5 compared to the control mice; D. Western Blot analysis of GATA1 protein expressions in CD71highTer119high cells. There was a significant decrease in the expression of GATA1 in the shRNA Rpl5 group compared to the control mice. In these experiments, GAPDH was used as a loading control, & they are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32309614), licensed under a CC-BY license. Not internally tested by Novus Biologicals.