RPL7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34111

Novus Biologicals, part of Bio-Techne
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NBP2-34111
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RPL7 Antibodies »

Product Summary for RPL7 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: VEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRKARRKLIY

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPL7 Antibody

Western Blot: RPL7 Antibody [NBP2-34111]

Western Blot: RPL7 Antibody [NBP2-34111]

Western Blot: RPL7 Antibody [NBP2-34111] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: RPL7 Antibody [NBP2-34111]

Immunocytochemistry/ Immunofluorescence: RPL7 Antibody [NBP2-34111]

Immunocytochemistry/Immunofluorescence: RPL7 Antibody [NBP2-34111] - Staining of human cell line A-431 shows localization to nucleoli, cytosol & endoplasmic reticulum.
Immunohistochemistry-Paraffin: RPL7 Antibody [NBP2-34111]

Immunohistochemistry-Paraffin: RPL7 Antibody [NBP2-34111]

Immunohistochemistry-Paraffin: RPL7 Antibody [NBP2-34111] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Applications for RPL7 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPL7

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Alternate Names

humL7-1,60S ribosomal protein L7, MGC117326, ribosomal protein L7

Gene Symbol

RPL7

UniProt

P18124 (Human)

Additional RPL7 Products

Product Documents for RPL7 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RPL7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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