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RPLP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81293

Novus Biologicals, part of Bio-Techne
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NBP1-81293-25ul
NBP1-81293

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MASVSELACIYSALILHDDEVTVTEDKINALIKAAGVNVEPFWPGLFAKALANVNIGSLICNVGAGGPAPAAGAAPAGGPAPSTAAAPAEEKKVEAKKEESEESDDDMG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPLP1 Antibody

Western Blot: RPLP1 Antibody [NBP1-81293]

Western Blot: RPLP1 Antibody [NBP1-81293]

Western Blot: RPLP1 Antibody [NBP1-81293] - Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: RPLP1 Antibody [NBP1-81293]

Immunocytochemistry/ Immunofluorescence: RPLP1 Antibody [NBP1-81293]

Immunocytochemistry/Immunofluorescence: RPLP1 Antibody [NBP1-81293] - Staining of human cell line U-2 OS shows localization to cytosol & endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RPLP1 Antibody [NBP1-81293]

Immunohistochemistry-Paraffin: RPLP1 Antibody [NBP1-81293]

Immunohistochemistry-Paraffin: RPLP1 Antibody [NBP1-81293] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.

Applications for RPLP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPLP1

RPLP1, also known as 60S acidic ribosomal protein P1, is a 12kDa 114 amino acid protein with a shorter 9kDa 89 isoform. The primary function of RPLP1 is to regulate the elongation step during protein synthesis. Current research is being conducted on RPLP1 in relation to the diseases: pandas, rhabdomyosarcoma, mouth disease, breast cancer and malaria. RPLP1 has been linked to the proteolysis, virulence, secretion, cell death and methylation pathways where it interacts with ASCC2, TPT1, NUDT3, NUDT21 and ANKRD13B.

Alternate Names

acidic ribosomal phosphoprotein P1, FLJ27448, MGC5215, P1,60S acidic ribosomal protein P1, ribosomal protein, large, P1, RPP1, RRP1

Gene Symbol

RPLP1

Additional RPLP1 Products

Product Documents for RPLP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RPLP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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