RPP30 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80658

Novus Biologicals, part of Bio-Techne
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NBP1-80658
NBP1-80658-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RPP30 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NVIISSAAERPLEIRGPYDVANLGLLFGLSESDAKAAVSTNCRAALLHGETRKTAFGIISTVKKPRPSEGDEDCLPASKKAKCEG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPP30 Antibody

Immunocytochemistry/ Immunofluorescence: RPP30 Antibody [NBP1-80658]

Immunocytochemistry/ Immunofluorescence: RPP30 Antibody [NBP1-80658]

Immunocytochemistry/Immunofluorescence: RPP30 Antibody [NBP1-80658] - Staining of human cell line U-251 MG shows localization to nucleus, nucleoli and microtubule ends.
Immunohistochemistry-Paraffin: RPP30 Antibody [NBP1-80658]

Immunohistochemistry-Paraffin: RPP30 Antibody [NBP1-80658]

Immunohistochemistry-Paraffin: RPP30 Antibody [NBP1-80658] - Staining in human testis and pancreas tissues using anti-RPP30 antibody. Corresponding RPP30 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: RPP30 Antibody [NBP1-80658]

Immunohistochemistry-Paraffin: RPP30 Antibody [NBP1-80658]

Immunohistochemistry-Paraffin: RPP30 Antibody [NBP1-80658] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts and Leydig cells.
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Applications for RPP30 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPP30

RPP30 is a component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends

Alternate Names

EC 3.1.26.5, FLJ38491, ribonuclease P (30kD) (RPP30), ribonuclease P protein subunit p30, ribonuclease P/MRP 30kDa subunit, RNase P subunit 2, RNaseP protein p30, RNASEP2, TSG15

Entrez Gene IDs

10556 (Human)

Gene Symbol

RPP30

UniProt

P78346 (Human)

Additional RPP30 Products

Product Documents for RPP30 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RPP30 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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