RPS17 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12556
![Immunoprecipitation: RPS17 Antibody [NBP3-12556]](https://resources.bio-techne.com/images/products/RPS17-Antibody-Immunoprecipitation-NBP3-12556-img0001.jpg "Immunoprecipitation: RPS17 Antibody [NBP3-12556]")
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for RPS17 Antibody
Immunogen
Produced in rabbits immunized with E. coli-derived Human RPS17 fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Protein A & Antigen Affinity purified
Protein A & Antigen Affinity purified
Scientific Data Images for RPS17 Antibody
![Western Blot: RPS17 Antibody [NBP3-12556]](https://resources.bio-techne.com/images/products/RPS17-Antibody-Western-Blot-NBP3-12556-img0004.jpg "Western Blot: RPS17 Antibody [NBP3-12556]")
Western Blot: RPS17 Antibody [NBP3-12556]
Western Blot: RPS17 Antibody [NBP3-12556] - Lane A: HeLa Whole Cell LysateLane B: NCI-H292 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:15 kDaObserved band size:15 kDa(We are unsure as to the identity of these extra bands.)![Immunocytochemistry/ Immunofluorescence: RPS17 Antibody [NBP3-12556]](https://resources.bio-techne.com/images/products/RPS17-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12556-img0003.jpg "Immunocytochemistry/ Immunofluorescence: RPS17 Antibody [NBP3-12556]")
Immunocytochemistry/ Immunofluorescence: RPS17 Antibody [NBP3-12556]
Immunocytochemistry/Immunofluorescence: RPS17 Antibody [NBP3-12556] - Staining of RPS17 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti- RPS17 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.![Immunohistochemistry-Paraffin: RPS17 Antibody [NBP3-12556]](https://resources.bio-techne.com/images/products/RPS17-Antibody-Immunohistochemistry-Paraffin-NBP3-12556-img0002.jpg "Immunohistochemistry-Paraffin: RPS17 Antibody [NBP3-12556]")
Immunohistochemistry-Paraffin: RPS17 Antibody [NBP3-12556]
Immunohistochemistry-Paraffin: RPS17 Antibody [NBP3-12556] - Staining of human RPS17 in human brain with rabbit polyclonal antibody at 1:1000 dilution.Applications for RPS17 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:500-1:2000
Immunohistochemistry-Paraffin
1:500-1:2000
Immunoprecipitation
1-5uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RPS17
Alternate Names
40S ribosomal protein S17, DBA4, ribosomal protein S17, RPS17L1RPS17L2MGC72007
Gene Symbol
RPS17
Additional RPS17 Products
Product Specific Notices for RPS17 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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