RPTOR Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24837

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-24837
NBP2-24837SS
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all RPTOR Antibodies »

Product Specifications

Immunogen

A portion of amino acids 900-950 of human Raptor was used as the immunogen for this antibody.

Reactivity Notes

The sequence used as immunogen is 86% homologous in rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPTOR Antibody

Western Blot: RPTOR Antibody [NBP2-24837]

Western Blot: RPTOR Antibody [NBP2-24837]

Western Blot: RPTOR Antibody [NBP2-24837] - Analysis of Raptor in A) human 293 and B) mouse NIH 3T3 lysate using Raptor antibody at 1:2000.
Immunocytochemistry/ Immunofluorescence: RPTOR Antibody [NBP2-24837]

Immunocytochemistry/ Immunofluorescence: RPTOR Antibody [NBP2-24837]

Immunocytochemistry/Immunofluorescence: RPTOR Antibody [NBP2-24837] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-RPTOR at a 1:100 overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: RPTOR Antibody [NBP2-24837]

Immunohistochemistry-Paraffin: RPTOR Antibody [NBP2-24837]

Immunohistochemistry-Paraffin: RPTOR Antibody [NBP2-24837] - Analysis of human small intestine using Raptor antibody at 1:100.

Applications for RPTOR Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1: 100

Western Blot

1: 1000-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera with 50 ul neat whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RPTOR

Raptor (regulatory associated protein of TOR) is a conserved 150 kDa adaptor protein composed of a unique conserved region, three HEAT repeats and seven WD-40 repeats (1). Raptor recruits mTOR substrates S6K1 (p70 S6 kinase) and 4E-BP1, and regulates mTOR kinase activity (2-3). Even though Raptor is a positive regulator of mTOR, it has been shown that under nutrient deprivation, the Raptor-mTOR association is stabilized in a manner that inhibits mTOR kinase activity (1). Raptor association with mTOR is required for efficient S6K1 and 4E0BP1 phosphorylation (4).

Long Name

Regulatory Associated Protein of mTOR

Alternate Names

KIAA1303, Raptor

Gene Symbol

RPTOR

Additional RPTOR Products

Product Documents for RPTOR Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RPTOR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.