RRAGB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85801

Novus Biologicals, part of Bio-Techne
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NBP1-85801
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RRAGB Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPDAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECSCF

Localization

Golgi apparatus & Vesicles

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RRAGB Antibody

Western Blot: RRAGB Antibody [NBP1-85801]

Western Blot: RRAGB Antibody [NBP1-85801]

Western Blot: RRAGB Antibody [NBP1-85801] - Analysis in human cell line EFO-21.
Immunocytochemistry/ Immunofluorescence: RRAGB Antibody [NBP1-85801]

Immunocytochemistry/ Immunofluorescence: RRAGB Antibody [NBP1-85801]

Immunocytochemistry/Immunofluorescence: RRAGB Antibody [NBP1-85801] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RRAGB Antibody [NBP1-85801]

Immunohistochemistry-Paraffin: RRAGB Antibody [NBP1-85801]

Immunohistochemistry-Paraffin: RRAGB Antibody [NBP1-85801] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Applications for RRAGB Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RRAGB

Ras-homologous GTPases constitute a large family of signal transducers that alternate between an activated, GTP-binding state and an inactivated, GDP-binding state. These proteins represent cellular switches that are operated by GTP-exchange factors and factors that stimulate their intrinsic GTPase activity. All GTPases of the Ras superfamily have in common the presence of six conserved motifs involved in GTP/GDP binding, three of which are phosphate-/magnesium-binding sites (PM1-PM3) and three of which are guanine nucleotide-binding sites (G1-G3). Transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

Alternate Names

bA465E19.1, GTP-binding protein ragB, Rag B, RAGB, Ras-related GTP binding B, ras-related GTP-binding protein B

Entrez Gene IDs

10325 (Human)

Gene Symbol

RRAGB

UniProt

Q5VZM2 (Human)

Additional RRAGB Products

Product Documents for RRAGB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RRAGB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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