Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RRN3 Antibodies »

Product Summary for RRN3 Antibody

Immunogen

The immunogen for this product maps to a region between residue 601 and 651 of human Transcription Initiation Factor IA using the numbering given in entry NP_060897.3 (GeneID 54700).

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RRN3 Antibody

Western Blot: RRN3 Antibody [NBP1-71822]

Western Blot: RRN3 Antibody [NBP1-71822]

Western Blot: RRN3 Antibody [NBP1-71822] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-TIF-IA antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. TIF-IA was also immunoprecipitated by another rabbit anti-TIF-IA antibody which recognizes an upstream epitope.
Western Blot: RRN3 Antibody [NBP1-71822]

Western Blot: RRN3 Antibody [NBP1-71822]

Western Blot: RRN3 Antibody [NBP1-71822] - 20ug of WCE of 293T or NIH cells from a review by a confirmed customer.

Applications for RRN3 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP1-71822 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RRN3

RRN3 is required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent

Alternate Names

DKFZp566E104, RNA polymerase I-specific transcription initiation factor RRN3, RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae), RRN3 RNA polymerase I transcription factor homolog (yeast), TIF-IA, TIFIAMGC104238, Transcription initiation factor IA, transcription initiation factor TIF-IA

Gene Symbol

RRN3

UniProt

NP_060897.3 (Human)

Additional RRN3 Products

Product Documents for RRN3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RRN3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.