RSK1 [p Thr359, p Ser363] Antibody (1B6)

Immunoprecipitation: RSK1 [p Thr359, p Ser363] Antibody (1B6) [NBP3-26394] -

Immunoprecipitation: RSK1 [p Thr359, p Ser363] Antibody (1B6) [NBP3-26394] - Immunoprecipitating RSK1 [p Thr359, p Ser363] in Hela whole cell lysate.
Lane 1: Rabbit control IgG (1ug) instead of NBP3-26394 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26394 (3ug) + Hela whole cell lysate (1mg).
Lane 3: Hela whole cell lysate (20ug).

Western Blot: RSK1 [p Thr359, p Ser363] Antibody (1B6) [NBP3-26394] -

Western Blot: RSK1 [p Thr359, p Ser363] Antibody (1B6) [NBP3-26394] - Positive Western Blot detected in HepG2 whole cell lysate (treated with EGF or not).
All lanes: RSK1 [p Thr359, p Ser363] Antibody at 0.69ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 90 KDa
Observed band size: 90 KDa

Immunocytochemistry/Immunofluorescence: RSK1 [p Thr359, p Ser363] Antibody (1B6) [NBP3-26394] -

Immunocytochemistry/Immunofluorescence: RSK1 [p Thr359, p Ser363] Antibody (1B6) [NBP3-26394] - Staining of Hela cells with RSK1 [p Thr359, p Ser363] Antibody (1B6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).