RSK1 [p Thr573] Antibody (JE50-12)

Western Blot: RSK1 [p Thr573] Antibody (JE50-12) [NBP3-32798]

Western Blot: RSK1 [p Thr573] Antibody (JE50-12) [NBP3-32798] - Western blot analysis of RSK1 on different lysates with Rabbit anti-RSK1 antibody (NBP3-32798) at 1/1,000 dilution.

Lane 1: K-562 cell lysate
Lane 2: K-562 treated with 500nM PMA for 15 minutes cell lysate
Lane 3: HeLa cell lysate
Lane 4: HeLa treated with 200nM PMA for 4 hours cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 83 kDa
Observed band size: 83 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32798) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.