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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Chicken (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human RPS6K1. The immunogen is located within amino acids 600 - 650 of RPS6K1. Amino Acid Squence: ANGPSDTPEEILTR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RSK1 Antibody - BSA Free

Western Blot: RSK1 AntibodyBSA Free [NBP1-76620]

Western Blot: RSK1 AntibodyBSA Free [NBP1-76620]

Western Blot: RSK1 Antibody [NBP1-76620] - Analysis of RSK1 in Jurkat cells with the RSK1 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: RSK1 Antibody - BSA Free [NBP1-76620]

Immunocytochemistry/ Immunofluorescence: RSK1 Antibody - BSA Free [NBP1-76620]

Immunocytochemistry/Immunofluorescence: RSK1 Antibody [NBP1-76620] - Immunofluorescence of RP S6 Kinase in Jurkat cells with RP S6 Kinase antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: RSK1 Antibody - BSA Free [NBP1-76620]

Immunocytochemistry/ Immunofluorescence: RSK1 Antibody - BSA Free [NBP1-76620]

Immunocytochemistry/Immunofluorescence: RSK1 Antibody [NBP1-76620] - Jurkat cells with RSK1 antibody at 10 ug/ml.

Applications for RSK1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 -20 ug/ml

Western Blot

2.5-10 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RSK1

Ribosomal protein S6 kinase 1 (RPS6K1) is the best characterized effector of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Nutrients and growth factors stimulate a complex including TOR, raptor (regulatory associated protein of TOR), and GbetaL to phosphorylate RPS6K1 and the eukaryotic initiation factor 4E binding protein (4EBP1), leading to increased protein synthesis and cell growth. RPS6K1 is thought to desensitize tissues to insulin as mice deficient in RPS6K1 have been shown to be hypersensitive to insulin and impervious to obesity-induced insulin resistance seen in wild type obese mice.

Long Name

Ribosomal Protein S6 Kinase I

Alternate Names

HU-1, MAPKAPL1A, RPS6KA1

Gene Symbol

RPS6KA1

Additional RSK1 Products

Product Documents for RSK1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RSK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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