RTEL1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22360

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Block/Neutralize, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.26 mg/ml

View all RTEL1 Antibodies »

Product Specifications

Immunogen

A genomic peptide made to an internal region of the human RTEL1 protein (within residues 800-1000). [Swiss-Prot Q9NZ71]

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RTEL1 Antibody - BSA Free

Western Blot: RTEL1 AntibodyBSA Free [NBP2-22360]

Western Blot: RTEL1 AntibodyBSA Free [NBP2-22360]

Western Blot: RTEL1 Antibody [NBP2-22360] - WB analysis of RTEL1 in HeLa cell lysate.
Immunocytochemistry/ Immunofluorescence: RTEL1 Antibody - BSA Free [NBP2-22360]

Immunocytochemistry/ Immunofluorescence: RTEL1 Antibody - BSA Free [NBP2-22360]

Immunocytochemistry/Immunofluorescence: RTEL1 Antibody [NBP2-22360] - RTEL1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: RTEL1 AntibodyBSA Free [NBP2-22360]

Western Blot: RTEL1 AntibodyBSA Free [NBP2-22360]

Western Blot: RTEL1 Antibody [NBP2-22360] - WB analysis of RTEL1 in mouse testis lysate.

Applications for RTEL1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250 - 1:500

Western Blot

1:5000 - 1:10000
Application Notes
In Immunohistochemistry and ICC/IF nuclear staining was observed. Formulin fixation is recommended for ICC/IF.

Reviewed Applications

Read 1 review rated 4 using NBP2-22360 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.26 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RTEL1

RTEL1 (Regulator of Telomere Length 1) is a nuclear iron-sulfur cluster containing ATP-dependent DNA helicase essential to genomic stability. RTEL1 contains 1 helicase ATP-binding domain and it belongs to helicase family, RAD3/XPD subfamily. Originally discovered in mouse, RTEL1 is a dominant factor controlling the regulation of telomeric length and genomic integrity, and recent evidence suggest that RTEL1 is preferentially recruited to, or exerts greater activity at genome's G-rich regions. In DNA double-strand breaks (DSBs) repair, RTEL1 disrupts D-loops in vitro and promotes synthesis-dependent strand annealing (SDSA) in vivo to direct DNA DSBs into non-crossover outcomes during mitotic repair. RTEL1 activity is also essential to meiotic recombination regulation as shown in C elegans. RTEL1's multiple roles in genomic stability maintenance and recombination regulation suggests it as a tumor suppressor. Indeed, RTEL1 mutation represents a risk factor for glioma and it is overexpressed in gastrointestinal cancers. Human RTEL1 deficiency caused Hoyeraal-Hreidarsson syndrome (HHS), a severe variant of Dyskeratosis Congenita (DC), characterized by early onset bone marrow failure, immunodeficiency and developmental defects.

Alternate Names

C20orf41, regulator of telomere elongation helicase 1, regulator of telomere length

Entrez Gene IDs

51750 (Human)

Gene Symbol

RTEL1

UniProt

Q9NZ71 (Human)

Additional RTEL1 Products

Product Documents for RTEL1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RTEL1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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