RTKN Antibody (2E5)

Novus Biologicals, part of Bio-Techne | Catalog # H00006242-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RTKN Antibodies »

Product Specifications

Immunogen

RTKN (NP_149035, 451 a.a. ~ 549 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VTDILTQREGARLETPPPWLAMFTDQPALPNPCSPASVAPAPDWTHPLPWGRPRTFSLDAVPPDHSPRARSVAPLPPQRSPRTRGLCSKGQPRTWLQSP

Specificity

RTKN (2E5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RTKN Antibody (2E5)

Western Blot: RTKN Antibody (2E5) [H00006242-M01]

Western Blot: RTKN Antibody (2E5) [H00006242-M01]

Western Blot: RTKN Antibody (2E5) [H00006242-M01] - RTKN monoclonal antibody (M01), clone 2E5 Analysis of RTKN expression in A-431.
Immunohistochemistry-Paraffin: RTKN Antibody (2E5) [H00006242-M01]

Immunohistochemistry-Paraffin: RTKN Antibody (2E5) [H00006242-M01]

Immunohistochemistry-Paraffin: RTKN Antibody (2E5) [H00006242-M01] - Analysis of monoclonal antibody to RTKN on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Sandwich ELISA: RTKN Antibody (2E5) [H00006242-M01] - Detection limit for recombinant GST tagged RTKN is approximately 0.3ng/ml as a capture antibody.

Applications for RTKN Antibody (2E5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA. ICC/IF usage was reported in scientific literature.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RTKN

This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

B5, rhotekin, RTKN1

Entrez Gene IDs

6242 (Human)

Gene Symbol

RTKN

OMIM

602288 (Human)

UniProt

NP_149035 (Human)

Additional RTKN Products

Product Documents for RTKN Antibody (2E5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RTKN Antibody (2E5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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