RTN1-A/NSP Antibody (MON161) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97678
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Hamster
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # MON161
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with a partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
This antibody exclusively recognizes the 135 kD RTN1-A/NSP protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues. The epitope of MON-161 is located between amino acid residues 174-337 of RTN1-A/NSP.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Applications for RTN1-A/NSP Antibody (MON161) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:50-1:100
Immunohistochemistry-Paraffin
1:50-1:100
Western Blot
1:50-1:500
Application Notes
This antibody is suitable for immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissue, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:50 - 1:100 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:50 - 1:500 for immunoblotting applications.
This antibody is CyTOF ready..
This antibody is CyTOF ready..
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS, pH 7.2
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RTN1-A/NSP
Long Name
Reticulon 1A/Neuroendocrine-specific Protein
Alternate Names
NSP-A, RTN1, RTN1A
Gene Symbol
RTN1
Additional RTN1-A/NSP Products
Product Documents for RTN1-A/NSP Antibody (MON161) - BSA Free
Product Specific Notices for RTN1-A/NSP Antibody (MON161) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...