Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Rubicon Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 922 and 972 of human Rubicon using the numbering given in entry NP_055502.1 (GeneID 9711).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rubicon Antibody

Western Blot: Rubicon Antibody [NBP1-30461]

Western Blot: Rubicon Antibody [NBP1-30461]

Western Blot: Rubicon Antibody [NBP1-30461] - Detection of Human Rubicon by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rubicon antibody NBP1-30461 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: Rubicon Antibody [NBP1-30461]

Immunoprecipitation: Rubicon Antibody [NBP1-30461]

Immunoprecipitation: Rubicon Antibody [NBP1-30461] - Detection of human Rubicon by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rubicon antibody NBP1-30461 (lot NBP1-30461-2) used for IP at 6 ug per reaction. Rubicon was also immunoprecipitated by a previous lot of this antibody (lot NBP1-30461-1) and another rabbit anti-Rubicon antibody. For blotting immunoprecipitated Rubicon, NBP1-30461 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Rubicon Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Rubicon

Long Name

Rubicon Autophagy Regulator

Alternate Names

KIAA0226, RUBCN

Gene Symbol

RUBCN

Additional Rubicon Products

Product Documents for Rubicon Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rubicon Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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