RUNX1T1/ETO Antibody (5A12)

Novus Biologicals, part of Bio-Techne | Catalog # H00000862-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5A12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RUNX1T1/ETO Antibodies »

Product Specifications

Immunogen

RUNX1T1 (NP_004340, 416 a.a. ~ 525 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EEIWKKAEEAVNEVKRQAMTELQKAVSEAERKAHDMITTERAKMERTVAEAKRQAAEDALAVINQQEDSSESCWNCGRKASETCSGCNTARYCGSFCQHKDWEKHHHICG

Specificity

RUNX1T1 - runt-related transcription factor 1; translocated to, 1 (cyclin D-related)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RUNX1T1/ETO Antibody (5A12)

Western Blot: RUNX1T1/ETO Antibody (5A12) [H00000862-M01]

Western Blot: RUNX1T1/ETO Antibody (5A12) [H00000862-M01]

Western Blot: RUNX1T1/ETO Antibody (5A12) [H00000862-M01] - Analysis of RUNX1T1 expression in transfected 293T cell line by RUNX1T1 monoclonal antibody (M01), clone 5A12.Lane 1: RUNX1T1 transfected lysate(67.566 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: RUNX1T1/ETO Antibody (5A12) [H00000862-M01]

Immunocytochemistry/ Immunofluorescence: RUNX1T1/ETO Antibody (5A12) [H00000862-M01]

Immunocytochemistry/Immunofluorescence: RUNX1T1/ETO Antibody (5A12) [H00000862-M01] - Analysis of monoclonal antibody to RUNX1T1 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: RUNX1T1/ETO Antibody (5A12) [H00000862-M01] - Detection limit for recombinant GST tagged RUNX1T1 is approximately 0.3ng/ml as a capture antibody.

Applications for RUNX1T1/ETO Antibody (5A12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RUNX1T1/ETO

The protein encoded by this gene is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Several transcript variants encoding multiple isoforms have been found for this gene.

Alternate Names

AML1T1protein CBFA2T1, CBFA2T1Cyclin-D-related protein, CDRMGC2796, core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclinD-related, DKFZp564B213, Eight twenty one protein, ETOFLJ33145, MTG8acute myelogenous leukemia 1 translocation 1, cyclin-D related, Protein ETO, Protein MTG8, runt-related transcription factor 1; translocated to, 1 (cyclin D-related), Zinc finger MYND domain-containing protein 2, ZMYND2myeloid translocation gene on 8q22

Entrez Gene IDs

862 (Human)

Gene Symbol

RUNX1T1

OMIM

133435 (Human)

UniProt

NP_004340 (Human)

Additional RUNX1T1/ETO Products

Product Documents for RUNX1T1/ETO Antibody (5A12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RUNX1T1/ETO Antibody (5A12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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