Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1H1

Concentration

1 mg/ml

View all RUNX1T1/ETO Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RUNX1T1(NP_783553) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RUNX1T1/ETO Antibody (OTI1H1)

Western Blot: RUNX1T1/ETO Antibody (OTI1H1) [NBP2-02023]

Western Blot: RUNX1T1/ETO Antibody (OTI1H1) [NBP2-02023]

Western Blot: RUNX1T1/ETO Antibody (1H1) [NBP2-02023] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUNX1T1/ETO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUNX1T1/ETO.
Immunocytochemistry/Immunofluorescence: RUNX1T1/ETO Antibody (1H1) [NBP2-02023] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RUNX1T1/ETO.
RUNX1T1/ETO Antibody (OTI1H1)

Western Blot: RUNX1T1/ETO Antibody (OTI1H1) [NBP2-02023] -

Western Blot: RUNX1T1/ETO Antibody (OTI1H1) [NBP2-02023] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-RUNX1T1 monoclonal antibody (1:200).
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Applications for RUNX1T1/ETO Antibody (OTI1H1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RUNX1T1/ETO

This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq].

Alternate Names

AML1T1protein CBFA2T1, CBFA2T1Cyclin-D-related protein, CDRMGC2796, core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclinD-related, DKFZp564B213, Eight twenty one protein, ETOFLJ33145, MTG8acute myelogenous leukemia 1 translocation 1, cyclin-D related, Protein ETO, Protein MTG8, runt-related transcription factor 1; translocated to, 1 (cyclin D-related), Zinc finger MYND domain-containing protein 2, ZMYND2myeloid translocation gene on 8q22

Entrez Gene IDs

862 (Human)

Gene Symbol

RUNX1T1

Additional RUNX1T1/ETO Products

Product Documents for RUNX1T1/ETO Antibody (OTI1H1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RUNX1T1/ETO Antibody (OTI1H1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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