RUVBL2 Antibody (OTI1A6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73957

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all RUVBL2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragmengt corresponding to amino acids 113-370 of human RUVBL2(NP_006657) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RUVBL2 Antibody (OTI1A6) - Azide and BSA Free

Western Blot: RUVBL2 Antibody (OTI1A6)Azide and BSA Free [NBP2-73957]

Western Blot: RUVBL2 Antibody (OTI1A6)Azide and BSA Free [NBP2-73957]

Western Blot: RUVBL2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-73957] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUVBL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUVBL2.
Immunocytochemistry/ Immunofluorescence: RUVBL2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-73957]

Immunocytochemistry/ Immunofluorescence: RUVBL2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-73957]

Immunocytochemistry/Immunofluorescence: RUVBL2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-73957] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RUVBL2.
Immunohistochemistry: RUVBL2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-73957]

Immunohistochemistry: RUVBL2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-73957]

Immunohistochemistry: RUVBL2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-73957] - Staining of paraffin-embedded Human endometrium tissue using anti-RUVBL2 mouse monoclonal antibody.
View 7 more images

Applications for RUVBL2 Antibody (OTI1A6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RUVBL2

RUVBL2 encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq]

Alternate Names

48 kDa TATA box-binding protein-interacting protein, 51 kDa erythrocyte cytosolic protein, EC 3.6.1, EC 3.6.4.12,48 kDa TBP-interacting protein, ECP51TIP49B, erythrocyte cytosolic protein, 51-KD, INO80 complex subunit J, INO80JTAP54-beta, Repressing pontin 52, REPTIN, Reptin 52, Reptin52, RuvB (E coli homolog)-like 2, ruvB-like 2, RuvB-like 2 (E. coli), Rvb2, TBP-interacting protein, 48-KD, TIH2, TIP48ECP-51, TIP49b, TIP60-associated protein 54-beta

Gene Symbol

RUVBL2

Additional RUVBL2 Products

Product Documents for RUVBL2 Antibody (OTI1A6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RUVBL2 Antibody (OTI1A6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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