RXR alpha/NR2B1 Antibody (6Y10M1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15667

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15667-100ul
NBP3-15667-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6Y10M1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RXR alpha/NR2B1 Antibodies »

Product Summary for RXR alpha/NR2B1 Antibody (6Y10M1)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RXR alpha/NR2B1 (P19793). MDTKHFLPLDFSTQVNSSLTSPTGRGSMAAPSLHPSLGPGIGSPGQLHSPISTLSSPINGMGPPFSVISSPMGPHSMSVPTTPTLGFSTGSPQLSSPMNP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RXR alpha/NR2B1 Antibody (6Y10M1)

Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667]

Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667]

Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667] - Western blot analysis of extracts of various cell lines, using RXR alpha/NR2B1 antibody (NBP3-15667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667]

Immunocytochemistry/ Immunofluorescence: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667]

Immunocytochemistry/Immunofluorescence: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667] - Immunofluorescence analysis of HeLa cells using RXR alpha/NR2B1 Rabbit mAb (NBP3-15667) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RXR alpha/NR2B1 antibody (NBP3-15667) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Applications for RXR alpha/NR2B1 Antibody (6Y10M1)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:500 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RXR alpha/NR2B1

The steroid receptor superfamily acts through direct association with DNA sequences known as hormone response elements (HREs) and bind DNA as either homo- or heterodimers. The promiscuous mediator of heterodimerization, RXR, is the receptor for 9-cis reti

Long Name

Retinoid X Receptor, alpha

Alternate Names

NR2B1, RXRA

Gene Symbol

RXRA

Additional RXR alpha/NR2B1 Products

Product Documents for RXR alpha/NR2B1 Antibody (6Y10M1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RXR alpha/NR2B1 Antibody (6Y10M1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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