Ryanodine Receptor Antibody (34C) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB300-543

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Amphibian, Bovine, Canine, Ferret, Fish, Mink, Primate, Rabbit, Sheep

Applications

Validated:

Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Block/Neutralize, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 34C

Format

BSA Free

Concentration

1 mg/ml

View all Ryanodine Receptor Antibodies »

Product Specifications

Immunogen

Partially purified chicken pectoral muscle ryanodine receptor.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

This antibody detects ryanodine receptor Ryanodine Receptor-1 and Ryanodine Receptor-2 isoforms in amphibian, chicken, bovine, canine, fish, human, non-human primate, mouse, rabbit, mink, rat and sheep tissues. In chicken, it detects the alpha, beta and cardiac isoforms. This antibody also detects Ryanodine Receptor-3 in mouse cells. In frog, it detects the alpha and beta isoforms and in fish it detects the alpha isoform.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

565 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ryanodine Receptor Antibody (34C) - BSA Free

Immunohistochemistry-Paraffin: Ryanodine Receptor Antibody (34C) - BSA Free [NB300-543]

Immunohistochemistry-Paraffin: Ryanodine Receptor Antibody (34C) - BSA Free [NB300-543]

Immunohistochemistry-Paraffin: Ryanodine Receptor Antibody (34C) [NB300-543] - Biopsies of deparaffinized Human skeletal muscle tissue.

Applications for Ryanodine Receptor Antibody (34C) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:1000

Immunohistochemistry-Paraffin

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:5000
Application Notes
May be useful in IF, IHC-Fr, IP, and WB. WB: Detects an approx. 565 kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. IHC: Staining of RyR in chicken brain results in intense staining of cerebral Purkinje neurons. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 15564379).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ryanodine Receptor

The Ryanodine Receptor (RyR) is the channel responsible for calcium release from muscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR. Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively.

Alternate Names

ARVC2, ARVD2, cardiac muscle ryanodine receptor-calcium release channel, cardiac-type ryanodine receptor, CCO, central core disease of muscle, islet-type ryanodine receptor, kidney-type ryanodine receptor, MHS, MHS1, PPP1R137, protein phosphatase 1, regulatory subunit 137, ryanodine receptor 1, ryanodine receptor 1 (skeletal), ryanodine receptor 2, ryanodine receptor 2 (cardiac), RYDR, RYR, RYR-1, RYR-2, sarcoplasmic reticulum calcium release channel, Skeletal muscle calcium release channel, skeletal muscle ryanodine receptor, SKRR, type 1-like ryanodine receptor, type 2 ryanodine receptor, VTSIP

Entrez Gene IDs

20190 (Mouse); 114207 (Rat)

Gene Symbol

RYR1

UniProt

Q90984 (Chicken)

Additional Ryanodine Receptor Products

Product Documents for Ryanodine Receptor Antibody (34C) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ryanodine Receptor Antibody (34C) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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