S Opsin Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20194
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Functional Assay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
A synthetic peptide from human OPSB conjugated to blue carrier protein was used as the antigen.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for S Opsin Antibody - Azide and BSA Free
![Western Blot: S Opsin AntibodyAzide and BSA Free [NBP1-20194]](https://resources.bio-techne.com/images/products/S-Opsin-Antibody-Western-Blot-NBP1-20194-img0003.jpg "Western Blot: S Opsin AntibodyAzide and BSA Free [NBP1-20194]")
Western Blot: S Opsin AntibodyAzide and BSA Free [NBP1-20194]
Western Blot: S Opsin Antibody [NBP1-20194] - WB on tissue lysates. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:500 dilution, incubated overnight at 4C.![Immunohistochemistry-Paraffin: S Opsin Antibody - Azide and BSA Free [NBP1-20194]](https://resources.bio-techne.com/images/products/S-Opsin-Antibody-Immunohistochemistry-Paraffin-NBP1-20194-img0002.jpg "Immunohistochemistry-Paraffin: S Opsin Antibody - Azide and BSA Free [NBP1-20194]")
Immunohistochemistry-Paraffin: S Opsin Antibody - Azide and BSA Free [NBP1-20194]
Immunohistochemistry-Paraffin: S Opsin Antibody [NBP1-20194] - Sections of mouse eye. Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.![Immunohistochemistry-Paraffin: S Opsin Antibody - Azide and BSA Free [NBP1-20194]](https://resources.bio-techne.com/images/products/S-Opsin-Antibody-Immunohistochemistry-Paraffin-NBP1-20194-img0001.jpg "Immunohistochemistry-Paraffin: S Opsin Antibody - Azide and BSA Free [NBP1-20194]")
Immunohistochemistry-Paraffin: S Opsin Antibody - Azide and BSA Free [NBP1-20194]
Immunohistochemistry-Paraffin: S Opsin Antibody [NBP1-20194] - Sections of mouse eye. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.Applications for S Opsin Antibody - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
10-50 ug/ml
Western Blot
10-50 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID:32716032).
Reviewed Applications
Read 1 review rated 5 using NBP1-20194 in the following applications:
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Reconstitution
Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.
Formulation
Lyophilized from PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: S Opsin
Alternate Names
BCPBlue cone photoreceptor pigmentBlue-sensitive opsin, BOPshort-wave-sensitive opsin 1, CBT, opsin 1 (cone pigments), short-wave-sensitive, s-opsin
Gene Symbol
OPN1SW
UniProt
Additional S Opsin Products
Product Documents for S Opsin Antibody - Azide and BSA Free
Product Specific Notices for S Opsin Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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