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S-Tag Epitope Tag Antibody (G10-C8-R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32923

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32923

Key Product Details

Species Reactivity

Validated:

Non-species specific

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # G10-C8-R

Concentration

1 mg/ml

Product Summary for S-Tag Epitope Tag Antibody (G10-C8-R)

Immunogen

Synthetic peptide CKETAAAKFERQHMDS-KLH.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for S-Tag Epitope Tag Antibody (G10-C8-R)

S-Tag Epitope Tag Antibody (G10-C8-R)

Western Blot: S-Tag Epitope Tag Antibody (G10-C8-R) [NBP3-32923]

Western Blot: S-Tag Epitope Tag Antibody (G10-C8-R) [NBP3-32923] - Western blot analysis of S-Tag Epitope Tag on S tag recombinant protein with Mouse anti-S-Tag Epitope Tag antibody (NBP3-32923) at 1/1,000 dilution.

Lysates/proteins at 50 ng/Lane.

Exposure time: 25 seconds; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32923) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for S-Tag Epitope Tag Antibody (G10-C8-R)

Application
Recommended Usage

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: S-Tag Epitope Tag

The S-Tag epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized S-Tag epitope represents the amino acid sequence KETAAAKFERQHMDS derived from pancreatic ribonuclease A.

Alternate Names

KETAAAKFERQHMDS, S 7 Epitope tag

Additional S-Tag Epitope Tag Products

Product Documents for S-Tag Epitope Tag Antibody (G10-C8-R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for S-Tag Epitope Tag Antibody (G10-C8-R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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