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Key Product Details

Species Reactivity

Epitope Tag

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Goats were immunized with S-Tag (KETAAAKFERQHMDS) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for S-Tag Epitope Tag Antibody - BSA Free

Western Blot: S-Tag Epitope Tag AntibodyBSA Free [NB600-529]

Western Blot: S-Tag Epitope Tag AntibodyBSA Free [NB600-529]

Western Blot: S-Tag Epitope Tag Antibody [NB600-529] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-S-Tag antibody NB600-529 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: S-Tag Epitope Tag AntibodyBSA Free [NB600-529]

Western Blot: S-Tag Epitope Tag AntibodyBSA Free [NB600-529]

Western Blot: S-Tag Epitope Tag Antibody [NB600-529] - Analysis using the HRP conjugate of NB600-529. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).

Applications for S-Tag Epitope Tag Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000- 1:30000

Immunocytochemistry/ Immunofluorescence

1:100-1:400

Immunoprecipitation

1 - 4 ug/mg lysate

Western Blot

1:1000 - 1:30000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: S-Tag Epitope Tag

S tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A.

Alternate Names

KETAAAKFERQHMDS, S 7 Epitope tag

Additional S-Tag Epitope Tag Products

Product Documents for S-Tag Epitope Tag Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for S-Tag Epitope Tag Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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