S100 calcium binding protein A14 Antibody (S100A14/7401) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21128
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # S100A14/7401
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant full-length human S100 calcium binding protein A14 protein
Localization
Cytoplasm.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Positive Controls: Human colon, thymus, kidney or liver.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images
S100 calcium binding protein A14 Antibody (S100A14/7401)
Formalin-fixed, paraffin-embedded human bladder stained with S100 calcium binding protein A14 antibody (S100A14/7401). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
S100 calcium binding protein A14 Antibody (S100A14/7401)
Formalin-fixed, paraffin-embedded human colon carcinoma stained with S100 calcium binding protein A14 antibody (S100A14/7401). Inset: PBS instead of primary antibody; secondary only negative control.
S100 calcium binding protein A14 Antibody (S100A14/7401)
Formalin-fixed, paraffin-embedded human normal colon stained with S100 calcium binding protein A14 antibody (S100A14/7401). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: S100 calcium binding protein A14
Alternate Names
protein S100-A14, S100 calcium binding protein A14, S100 calcium-binding protein A14, S100A15BCMP84breast cancer membrane protein 84, S114
Gene Symbol
S100A14
Additional S100 calcium binding protein A14 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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