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S100A11 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99718

Novus Biologicals, part of Bio-Techne
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NBP2-99718-100ul
NBP2-99718-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human S100A11 (Accession#: NP_005611.1; Met1-Thr105)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for S100A11 Antibody

Western Blot: S100A11 Antibody [NBP2-99718]

Western Blot: S100A11 Antibody [NBP2-99718]

Western Blot: S100A11 Antibody [NBP2-99718] - Anti-S100A11 rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: SKBR3 Whole Cell Lysate. Lane C: PC-3 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 11 kDa. Observed band size: 11 kDa
Immunoprecipitation: S100A11 Antibody [NBP2-99718]

Immunoprecipitation: S100A11 Antibody [NBP2-99718]

Immunoprecipitation: S100A11 Antibody [NBP2-99718] - S100A11 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. 2 ul anti-S100A11 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-S100A11 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 11 kDa. Observed band size: 11 kDa

Applications for S100A11 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: S100A11

S100A11 is encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis.

Long Name

S100 Calcium Binding Protein A11

Alternate Names

Calgizzarin, MLN70, S100C

Gene Symbol

S100A11

Additional S100A11 Products

Product Documents for S100A11 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for S100A11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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