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S100A4 Antibody (S100A4/1481) [Alexa Fluor® 488]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54580AF488

Novus Biologicals, part of Bio-Techne
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NBP2-54580AF488

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

CyTOF-ready, ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # S100A4/1481

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary) (Uniprot: P26447)

Localization

Nucleus, Cytoplasm

Marker

Marker of Tumor Metastasis

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for S100A4 Antibody (S100A4/1481) [Alexa Fluor® 488]

S100A4 Antibody (S100A4/1481) [Alexa Fluor® 488] [NBP2-54580AF488] - 3 tissue arrays with 104 invasive ductal carcinoma tissue sections with metastatic carcinomas, 21 invasive ductal carcinoma tissue sections, & 8 normal tissue sections. 33 carcinoma tissue sections were -ve for estrogen & progesterone receptor; don't overexpress Her2neu receptor (triple -ve breast cancer, TNBC). (A) CYR61 &/or S100A4 expression analysis in 123 invasive ductal carcinoma tissue & lymph node sections of 104 patients (B); representative ICC/IF stains analyzed with 100x oil objective. (C) From 123 invasive ductal carcinomas tissue sections, 33 stated as being TNBC. (D) Normal breast tissue sections (n = 8) analyzed for CYR61 &/ or S100A4 expression using ICC/IF. Scale bar: 20 um. Image collected & cropped by CiteAb from the following publication (https://www.frontiersin.org/article/10.3389/fonc.2019.01074/full), licensed under a CC-BY license.

Applications for S100A4 Antibody (S100A4/1481) [Alexa Fluor® 488]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Protein Array

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: S100A4

S100A4 is encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Long Name

S100 Calcium Binding Protein A4

Alternate Names

18A2, 42A, CAPL, MTS1, P9KA, PEL98

Gene Symbol

S100A4

Additional S100A4 Products

Product Documents for S100A4 Antibody (S100A4/1481) [Alexa Fluor® 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for S100A4 Antibody (S100A4/1481) [Alexa Fluor® 488]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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