S100A8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90314

Novus Biologicals, part of Bio-Techne
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NBP1-90314
NBP1-90314-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all S100A8 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LTELEKALNSIIDVYHKYSLIKGNFHAVYRDDLKKLLETECPQYIRKKGADVWFKELDINTDGAVNFQEFLILVIKM

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25561705).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for S100A8 Antibody

Immunohistochemistry-Paraffin: S100A8 Antibody [NBP1-90314]

Immunohistochemistry-Paraffin: S100A8 Antibody [NBP1-90314]

Immunohistochemistry-Paraffin: S100A8 Antibody [NBP1-90314] - Staining in human bone marrow and duodenum tissues using anti-S100A8 antibody. Corresponding S100A8 RNA-seq data are presented for the same tissues.
Western Blot: S100A8 Antibody [NBP1-90314]

Western Blot: S100A8 Antibody [NBP1-90314]

Western Blot: S100A8 Antibody [NBP1-90314] - Analysis in human cell line SK-BR-3.
Immunocytochemistry/ Immunofluorescence: S100A8 Antibody [NBP1-90314]

Immunocytochemistry/ Immunofluorescence: S100A8 Antibody [NBP1-90314]

Immunocytochemistry/Immunofluorescence: S100A8 Antibody [NBP1-90314] - Staining of human cell line HaCaT shows localization to intermediate filaments. Antibody staining is shown in green.
View 2 more images

Applications for S100A8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: S100A8

MRP8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

Long Name

S100 Calcium Binding Protein A8

Alternate Names

Calgranulin A, CFAG, MRP8

Gene Symbol

S100A8

Additional S100A8 Products

Product Documents for S100A8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for S100A8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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