S100A9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89360

Novus Biologicals, part of Bio-Techne
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NBP1-89360
NBP1-89360-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all S100A9 Antibodies »

Product Summary for S100A9 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CKMSQLERNIETIINTFHQYSVKLGHPDTLNQGEFKELVRKDLQNFLKKENKNEKVIEHIMEDLDTNADKQLSFEEFIMLMARLTWASHEKMHEGDEG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for S100A9 Antibody

Immunohistochemistry-Paraffin: S100A9 Antibody [NBP1-89360]

Immunohistochemistry-Paraffin: S100A9 Antibody [NBP1-89360]

Immunohistochemistry-Paraffin: S100A9 Antibody [NBP1-89360] - Analysis in human esophagus and stomach tissues. Corresponding S100A9 RNA-seq data are presented for the same tissues.
Western Blot: S100A9 Antibody [NBP1-89360]

Western Blot: S100A9 Antibody [NBP1-89360]

Western Blot: S100A9 Antibody [NBP1-89360] - Analysis in human cell line SK-BR-3.
Immunohistochemistry-Paraffin: S100A9 Antibody [NBP1-89360]

Immunohistochemistry-Paraffin: S100A9 Antibody [NBP1-89360]

Immunohistochemistry-Paraffin: S100A9 Antibody [NBP1-89360] - Staining of human stomach shows no positivity in glandular cells as expected.
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Applications for S100A9 Antibody

Application
Recommended Usage

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Simple Western

1:20

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: S100A9

S100A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A9 may function in the inhibition of casein kinase, and altered expression is associated with the disease cystic fibrosis. Overexpression of S100A9 has also recently been linked to a poor prognosis with invasive ductal carcinoma of the breast.

Long Name

S100 Calcium Binding Protein A9

Alternate Names

Calgranulin B, MRP-14

Gene Symbol

S100A9

Additional S100A9 Products

Product Documents for S100A9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for S100A9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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