S1P1/EDG-1 Antibody (4F2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15216

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15216
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4F2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all S1P1/EDG-1 Antibodies »

Product Summary for S1P1/EDG-1 Antibody (4F2)

Immunogen

A synthesized peptide derived from human S1P1/EDG-1. UniProt ID (P21453).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for S1P1/EDG-1 Antibody (4F2)

Western Blot: S1P1/EDG-1 Antibody (4F2) [NBP3-15216]

Western Blot: S1P1/EDG-1 Antibody (4F2) [NBP3-15216]

Western Blot: S1P1/EDG-1 Antibody (4F2) [NBP3-15216] - Positive WB detected in: SH-SY5Y whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, U251 whole cell lysate. All lanes: S1P1/EDG-1 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43 KDa. Observed band size: 43 KDa.

Applications for S1P1/EDG-1 Antibody (4F2)

Application
Recommended Usage

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: S1P1/EDG-1

EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50 kDa] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes. EBP 50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP 50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP 50 is found exclusively at the apical membranes of epithelial cells.

Long Name

Endothelial Differentiation Gene 1

Alternate Names

CD363, EDG1, S1PR1

Gene Symbol

S1PR1

Additional S1P1/EDG-1 Products

Product Documents for S1P1/EDG-1 Antibody (4F2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for S1P1/EDG-1 Antibody (4F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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