S5a/Angiocidin Antibody (3C9)

Novus Biologicals, part of Bio-Techne | Catalog # H00005710-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3C9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all S5a/Angiocidin Antibodies »

Product Specifications

Immunogen

PSMD4 (NP_002801, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRII

Specificity

PSMD4 - proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (3C9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for S5a/Angiocidin Antibody (3C9)

Western Blot: S5a/Angiocidin Antibody (3C9) [H00005710-M01]

Western Blot: S5a/Angiocidin Antibody (3C9) [H00005710-M01]

Western Blot: S5a/Angiocidin Antibody (3C9) [H00005710-M01] - Analysis of PSMD4 expression in transfected 293T cell line by PSMD4 monoclonal antibody (M01), clone 3C9.Lane 1: PSMD4 transfected lysate(40.7 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: S5a/Angiocidin Antibody (3C9) [H00005710-M01] - Detection limit for recombinant GST tagged PSMD4 is 0.3 ng/ml as a capture antibody.

Applications for S5a/Angiocidin Antibody (3C9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: S5a/Angiocidin

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21. [provided by RefSeq]

Long Name

26S Proteasome Regulatory Subunit S5a

Alternate Names

Angiocidin, ASF, Macropain, Mcb1, PSMD4, pUB-R5, Rpn10, S5a

Entrez Gene IDs

5710 (Human)

Gene Symbol

PSMD4

UniProt

NP_002801 (Human)

Additional S5a/Angiocidin Products

Product Documents for S5a/Angiocidin Antibody (3C9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for S5a/Angiocidin Antibody (3C9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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